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e faecalis atcc 47077 strr  (ATCC)


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    ATCC e faecalis atcc 47077 strr
    E Faecalis Atcc 47077 Strr, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 228 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+faecalis+atcc+47077+strr/Enterococcus+faecalis%3B+OG1RF/pm36445841__es2c05349_si_001-114-78-80
    Average 96 stars, based on 228 article reviews
    e faecalis atcc 47077 strr - by Bioz Stars, 2026-10
    96/100 stars

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    Bacteria:

    Article Title: Bisphenols Promote the Pheromone-Responsive Plasmid-Mediated Conjugative Transfer of Antibiotic Resistance Genes in Enterococcus faecalis .
    Article Snippet: .. S19 S20 Figure.S10 Standard curve corresponding to the log of the copy number of each primer gene and cycle threshold (a:ccfA; b:prgQ; c:prgA; d:prgB; e:prgX; f:prgY; g:prgZ; h:pcfT; i:pcfG; j:pcfY; k:16s rRNA gene) S21 Table S1 List of bacteria and plasmids Strains or plasmids Description Antibiotic type and working concentration. pCF10 pheromone-responsive plasmid TetR(10 mg/L) pK18mobsacB Widely used gene knockout vector, KanR KanR(50 mg/L) RP4 IncPα plasmid KanR(60 mg/L), TetR(40 mg/L), AmpR(80 mg/L) OG1RS the mutant strain of E. faecalis ATCC 47077 StrR(3 g/L), RifR(50 mg/L) OG1RF(pCF10) E. faecalis ATCC 47077 containing pCF10 plasmid TetR(10 mg/L), RifR(50 mg/L) OG1RSΔccfA E. faecalis ATCC 47077 containing pCF10 plasmid ccfA deletion mutants StrR(3 g/L), RifR(50 mg/L), KanR(50 mg/L) DH5α E. coli commonly used for plasmid cloning / K12 E. coli ATCC 47076,host of RP4, RifR RifR(100 mg/L) HB101(RP4) E. coli HB101containing RP4 plasmid KanR(60 mg/L), TetR(40 mg/L), AmpR(80 mg/L) S22 Table S2 Sequence of primers Gene name Primer Sequence( 5’ to 3’) Length of the product(bp) ccfA F:AGATATACGAAGGCCCGCTGAA 243 R: AATTAGTTATCCGCTGCTCCTGA prgQ F:TATAGGAGGGGTGTAAATGAAAAC 143 R: CCGTCCTATGGCAGTCG prgA F:GTTTGATGATGGCCGCTTAGGTT R:CACTTCGCCGTTTTGGTATTGACT 175 prgB F:AGCGGATGGAAAATTTTACTCACC R: GCGCACTAGATACAGGCACATTA 197 prgX F: AATGTTTTAAATCTGAAGTA 217 R: ACAAAAAGCGTAAATGA prgY F: TTTGGAAGGTGCGGAAGAATC 202 R: GTAAATGCGCCTGCCCAATA prgZ F: GACTTTGACTGCAGGGACACC 251 R: AGCGCCATCTCTAATCACAAT pcfG F: GAACCATGGCAACTGTCTCA 179 R: GCATCCTTGTCGCTCATAAA pcfT F: GCCACTTTGCTATTATCAGGA 224 R: GTTTTCGGCTTTACAGTCTCG pcfY F: AGATAATGCCCTAACCTTTGAGC 245 R: TCTTTTCTACTTCCCGCATACTGT 16s rRNA F: GCGGCGTGCCTAATACA 219 R: CCGCGGGTCCATCC S23 Table S3 Mass spectrum parameters Conditions Parameters Source temperature(°C) 150 Desolvent rate(L/h) 1000 Desolvent temperature(°C) 500 Parent(m/z) 790.8149 Daughter(m/z) 314.3200and 409.3026 Cone energy(V) 16 and 18 Collision energy(V) 36 and 26 ..

    Concentration Assay:

    Article Title: Bisphenols Promote the Pheromone-Responsive Plasmid-Mediated Conjugative Transfer of Antibiotic Resistance Genes in Enterococcus faecalis .
    Article Snippet: .. S19 S20 Figure.S10 Standard curve corresponding to the log of the copy number of each primer gene and cycle threshold (a:ccfA; b:prgQ; c:prgA; d:prgB; e:prgX; f:prgY; g:prgZ; h:pcfT; i:pcfG; j:pcfY; k:16s rRNA gene) S21 Table S1 List of bacteria and plasmids Strains or plasmids Description Antibiotic type and working concentration. pCF10 pheromone-responsive plasmid TetR(10 mg/L) pK18mobsacB Widely used gene knockout vector, KanR KanR(50 mg/L) RP4 IncPα plasmid KanR(60 mg/L), TetR(40 mg/L), AmpR(80 mg/L) OG1RS the mutant strain of E. faecalis ATCC 47077 StrR(3 g/L), RifR(50 mg/L) OG1RF(pCF10) E. faecalis ATCC 47077 containing pCF10 plasmid TetR(10 mg/L), RifR(50 mg/L) OG1RSΔccfA E. faecalis ATCC 47077 containing pCF10 plasmid ccfA deletion mutants StrR(3 g/L), RifR(50 mg/L), KanR(50 mg/L) DH5α E. coli commonly used for plasmid cloning / K12 E. coli ATCC 47076,host of RP4, RifR RifR(100 mg/L) HB101(RP4) E. coli HB101containing RP4 plasmid KanR(60 mg/L), TetR(40 mg/L), AmpR(80 mg/L) S22 Table S2 Sequence of primers Gene name Primer Sequence( 5’ to 3’) Length of the product(bp) ccfA F:AGATATACGAAGGCCCGCTGAA 243 R: AATTAGTTATCCGCTGCTCCTGA prgQ F:TATAGGAGGGGTGTAAATGAAAAC 143 R: CCGTCCTATGGCAGTCG prgA F:GTTTGATGATGGCCGCTTAGGTT R:CACTTCGCCGTTTTGGTATTGACT 175 prgB F:AGCGGATGGAAAATTTTACTCACC R: GCGCACTAGATACAGGCACATTA 197 prgX F: AATGTTTTAAATCTGAAGTA 217 R: ACAAAAAGCGTAAATGA prgY F: TTTGGAAGGTGCGGAAGAATC 202 R: GTAAATGCGCCTGCCCAATA prgZ F: GACTTTGACTGCAGGGACACC 251 R: AGCGCCATCTCTAATCACAAT pcfG F: GAACCATGGCAACTGTCTCA 179 R: GCATCCTTGTCGCTCATAAA pcfT F: GCCACTTTGCTATTATCAGGA 224 R: GTTTTCGGCTTTACAGTCTCG pcfY F: AGATAATGCCCTAACCTTTGAGC 245 R: TCTTTTCTACTTCCCGCATACTGT 16s rRNA F: GCGGCGTGCCTAATACA 219 R: CCGCGGGTCCATCC S23 Table S3 Mass spectrum parameters Conditions Parameters Source temperature(°C) 150 Desolvent rate(L/h) 1000 Desolvent temperature(°C) 500 Parent(m/z) 790.8149 Daughter(m/z) 314.3200and 409.3026 Cone energy(V) 16 and 18 Collision energy(V) 36 and 26 ..

    Plasmid Preparation:

    Article Title: Bisphenols Promote the Pheromone-Responsive Plasmid-Mediated Conjugative Transfer of Antibiotic Resistance Genes in Enterococcus faecalis .
    Article Snippet: .. S19 S20 Figure.S10 Standard curve corresponding to the log of the copy number of each primer gene and cycle threshold (a:ccfA; b:prgQ; c:prgA; d:prgB; e:prgX; f:prgY; g:prgZ; h:pcfT; i:pcfG; j:pcfY; k:16s rRNA gene) S21 Table S1 List of bacteria and plasmids Strains or plasmids Description Antibiotic type and working concentration. pCF10 pheromone-responsive plasmid TetR(10 mg/L) pK18mobsacB Widely used gene knockout vector, KanR KanR(50 mg/L) RP4 IncPα plasmid KanR(60 mg/L), TetR(40 mg/L), AmpR(80 mg/L) OG1RS the mutant strain of E. faecalis ATCC 47077 StrR(3 g/L), RifR(50 mg/L) OG1RF(pCF10) E. faecalis ATCC 47077 containing pCF10 plasmid TetR(10 mg/L), RifR(50 mg/L) OG1RSΔccfA E. faecalis ATCC 47077 containing pCF10 plasmid ccfA deletion mutants StrR(3 g/L), RifR(50 mg/L), KanR(50 mg/L) DH5α E. coli commonly used for plasmid cloning / K12 E. coli ATCC 47076,host of RP4, RifR RifR(100 mg/L) HB101(RP4) E. coli HB101containing RP4 plasmid KanR(60 mg/L), TetR(40 mg/L), AmpR(80 mg/L) S22 Table S2 Sequence of primers Gene name Primer Sequence( 5’ to 3’) Length of the product(bp) ccfA F:AGATATACGAAGGCCCGCTGAA 243 R: AATTAGTTATCCGCTGCTCCTGA prgQ F:TATAGGAGGGGTGTAAATGAAAAC 143 R: CCGTCCTATGGCAGTCG prgA F:GTTTGATGATGGCCGCTTAGGTT R:CACTTCGCCGTTTTGGTATTGACT 175 prgB F:AGCGGATGGAAAATTTTACTCACC R: GCGCACTAGATACAGGCACATTA 197 prgX F: AATGTTTTAAATCTGAAGTA 217 R: ACAAAAAGCGTAAATGA prgY F: TTTGGAAGGTGCGGAAGAATC 202 R: GTAAATGCGCCTGCCCAATA prgZ F: GACTTTGACTGCAGGGACACC 251 R: AGCGCCATCTCTAATCACAAT pcfG F: GAACCATGGCAACTGTCTCA 179 R: GCATCCTTGTCGCTCATAAA pcfT F: GCCACTTTGCTATTATCAGGA 224 R: GTTTTCGGCTTTACAGTCTCG pcfY F: AGATAATGCCCTAACCTTTGAGC 245 R: TCTTTTCTACTTCCCGCATACTGT 16s rRNA F: GCGGCGTGCCTAATACA 219 R: CCGCGGGTCCATCC S23 Table S3 Mass spectrum parameters Conditions Parameters Source temperature(°C) 150 Desolvent rate(L/h) 1000 Desolvent temperature(°C) 500 Parent(m/z) 790.8149 Daughter(m/z) 314.3200and 409.3026 Cone energy(V) 16 and 18 Collision energy(V) 36 and 26 ..

    Gene Knockout:

    Article Title: Bisphenols Promote the Pheromone-Responsive Plasmid-Mediated Conjugative Transfer of Antibiotic Resistance Genes in Enterococcus faecalis .
    Article Snippet: .. S19 S20 Figure.S10 Standard curve corresponding to the log of the copy number of each primer gene and cycle threshold (a:ccfA; b:prgQ; c:prgA; d:prgB; e:prgX; f:prgY; g:prgZ; h:pcfT; i:pcfG; j:pcfY; k:16s rRNA gene) S21 Table S1 List of bacteria and plasmids Strains or plasmids Description Antibiotic type and working concentration. pCF10 pheromone-responsive plasmid TetR(10 mg/L) pK18mobsacB Widely used gene knockout vector, KanR KanR(50 mg/L) RP4 IncPα plasmid KanR(60 mg/L), TetR(40 mg/L), AmpR(80 mg/L) OG1RS the mutant strain of E. faecalis ATCC 47077 StrR(3 g/L), RifR(50 mg/L) OG1RF(pCF10) E. faecalis ATCC 47077 containing pCF10 plasmid TetR(10 mg/L), RifR(50 mg/L) OG1RSΔccfA E. faecalis ATCC 47077 containing pCF10 plasmid ccfA deletion mutants StrR(3 g/L), RifR(50 mg/L), KanR(50 mg/L) DH5α E. coli commonly used for plasmid cloning / K12 E. coli ATCC 47076,host of RP4, RifR RifR(100 mg/L) HB101(RP4) E. coli HB101containing RP4 plasmid KanR(60 mg/L), TetR(40 mg/L), AmpR(80 mg/L) S22 Table S2 Sequence of primers Gene name Primer Sequence( 5’ to 3’) Length of the product(bp) ccfA F:AGATATACGAAGGCCCGCTGAA 243 R: AATTAGTTATCCGCTGCTCCTGA prgQ F:TATAGGAGGGGTGTAAATGAAAAC 143 R: CCGTCCTATGGCAGTCG prgA F:GTTTGATGATGGCCGCTTAGGTT R:CACTTCGCCGTTTTGGTATTGACT 175 prgB F:AGCGGATGGAAAATTTTACTCACC R: GCGCACTAGATACAGGCACATTA 197 prgX F: AATGTTTTAAATCTGAAGTA 217 R: ACAAAAAGCGTAAATGA prgY F: TTTGGAAGGTGCGGAAGAATC 202 R: GTAAATGCGCCTGCCCAATA prgZ F: GACTTTGACTGCAGGGACACC 251 R: AGCGCCATCTCTAATCACAAT pcfG F: GAACCATGGCAACTGTCTCA 179 R: GCATCCTTGTCGCTCATAAA pcfT F: GCCACTTTGCTATTATCAGGA 224 R: GTTTTCGGCTTTACAGTCTCG pcfY F: AGATAATGCCCTAACCTTTGAGC 245 R: TCTTTTCTACTTCCCGCATACTGT 16s rRNA F: GCGGCGTGCCTAATACA 219 R: CCGCGGGTCCATCC S23 Table S3 Mass spectrum parameters Conditions Parameters Source temperature(°C) 150 Desolvent rate(L/h) 1000 Desolvent temperature(°C) 500 Parent(m/z) 790.8149 Daughter(m/z) 314.3200and 409.3026 Cone energy(V) 16 and 18 Collision energy(V) 36 and 26 ..

    Mutagenesis:

    Article Title: Bisphenols Promote the Pheromone-Responsive Plasmid-Mediated Conjugative Transfer of Antibiotic Resistance Genes in Enterococcus faecalis .
    Article Snippet: .. S19 S20 Figure.S10 Standard curve corresponding to the log of the copy number of each primer gene and cycle threshold (a:ccfA; b:prgQ; c:prgA; d:prgB; e:prgX; f:prgY; g:prgZ; h:pcfT; i:pcfG; j:pcfY; k:16s rRNA gene) S21 Table S1 List of bacteria and plasmids Strains or plasmids Description Antibiotic type and working concentration. pCF10 pheromone-responsive plasmid TetR(10 mg/L) pK18mobsacB Widely used gene knockout vector, KanR KanR(50 mg/L) RP4 IncPα plasmid KanR(60 mg/L), TetR(40 mg/L), AmpR(80 mg/L) OG1RS the mutant strain of E. faecalis ATCC 47077 StrR(3 g/L), RifR(50 mg/L) OG1RF(pCF10) E. faecalis ATCC 47077 containing pCF10 plasmid TetR(10 mg/L), RifR(50 mg/L) OG1RSΔccfA E. faecalis ATCC 47077 containing pCF10 plasmid ccfA deletion mutants StrR(3 g/L), RifR(50 mg/L), KanR(50 mg/L) DH5α E. coli commonly used for plasmid cloning / K12 E. coli ATCC 47076,host of RP4, RifR RifR(100 mg/L) HB101(RP4) E. coli HB101containing RP4 plasmid KanR(60 mg/L), TetR(40 mg/L), AmpR(80 mg/L) S22 Table S2 Sequence of primers Gene name Primer Sequence( 5’ to 3’) Length of the product(bp) ccfA F:AGATATACGAAGGCCCGCTGAA 243 R: AATTAGTTATCCGCTGCTCCTGA prgQ F:TATAGGAGGGGTGTAAATGAAAAC 143 R: CCGTCCTATGGCAGTCG prgA F:GTTTGATGATGGCCGCTTAGGTT R:CACTTCGCCGTTTTGGTATTGACT 175 prgB F:AGCGGATGGAAAATTTTACTCACC R: GCGCACTAGATACAGGCACATTA 197 prgX F: AATGTTTTAAATCTGAAGTA 217 R: ACAAAAAGCGTAAATGA prgY F: TTTGGAAGGTGCGGAAGAATC 202 R: GTAAATGCGCCTGCCCAATA prgZ F: GACTTTGACTGCAGGGACACC 251 R: AGCGCCATCTCTAATCACAAT pcfG F: GAACCATGGCAACTGTCTCA 179 R: GCATCCTTGTCGCTCATAAA pcfT F: GCCACTTTGCTATTATCAGGA 224 R: GTTTTCGGCTTTACAGTCTCG pcfY F: AGATAATGCCCTAACCTTTGAGC 245 R: TCTTTTCTACTTCCCGCATACTGT 16s rRNA F: GCGGCGTGCCTAATACA 219 R: CCGCGGGTCCATCC S23 Table S3 Mass spectrum parameters Conditions Parameters Source temperature(°C) 150 Desolvent rate(L/h) 1000 Desolvent temperature(°C) 500 Parent(m/z) 790.8149 Daughter(m/z) 314.3200and 409.3026 Cone energy(V) 16 and 18 Collision energy(V) 36 and 26 ..

    Cloning:

    Article Title: Bisphenols Promote the Pheromone-Responsive Plasmid-Mediated Conjugative Transfer of Antibiotic Resistance Genes in Enterococcus faecalis .
    Article Snippet: .. S19 S20 Figure.S10 Standard curve corresponding to the log of the copy number of each primer gene and cycle threshold (a:ccfA; b:prgQ; c:prgA; d:prgB; e:prgX; f:prgY; g:prgZ; h:pcfT; i:pcfG; j:pcfY; k:16s rRNA gene) S21 Table S1 List of bacteria and plasmids Strains or plasmids Description Antibiotic type and working concentration. pCF10 pheromone-responsive plasmid TetR(10 mg/L) pK18mobsacB Widely used gene knockout vector, KanR KanR(50 mg/L) RP4 IncPα plasmid KanR(60 mg/L), TetR(40 mg/L), AmpR(80 mg/L) OG1RS the mutant strain of E. faecalis ATCC 47077 StrR(3 g/L), RifR(50 mg/L) OG1RF(pCF10) E. faecalis ATCC 47077 containing pCF10 plasmid TetR(10 mg/L), RifR(50 mg/L) OG1RSΔccfA E. faecalis ATCC 47077 containing pCF10 plasmid ccfA deletion mutants StrR(3 g/L), RifR(50 mg/L), KanR(50 mg/L) DH5α E. coli commonly used for plasmid cloning / K12 E. coli ATCC 47076,host of RP4, RifR RifR(100 mg/L) HB101(RP4) E. coli HB101containing RP4 plasmid KanR(60 mg/L), TetR(40 mg/L), AmpR(80 mg/L) S22 Table S2 Sequence of primers Gene name Primer Sequence( 5’ to 3’) Length of the product(bp) ccfA F:AGATATACGAAGGCCCGCTGAA 243 R: AATTAGTTATCCGCTGCTCCTGA prgQ F:TATAGGAGGGGTGTAAATGAAAAC 143 R: CCGTCCTATGGCAGTCG prgA F:GTTTGATGATGGCCGCTTAGGTT R:CACTTCGCCGTTTTGGTATTGACT 175 prgB F:AGCGGATGGAAAATTTTACTCACC R: GCGCACTAGATACAGGCACATTA 197 prgX F: AATGTTTTAAATCTGAAGTA 217 R: ACAAAAAGCGTAAATGA prgY F: TTTGGAAGGTGCGGAAGAATC 202 R: GTAAATGCGCCTGCCCAATA prgZ F: GACTTTGACTGCAGGGACACC 251 R: AGCGCCATCTCTAATCACAAT pcfG F: GAACCATGGCAACTGTCTCA 179 R: GCATCCTTGTCGCTCATAAA pcfT F: GCCACTTTGCTATTATCAGGA 224 R: GTTTTCGGCTTTACAGTCTCG pcfY F: AGATAATGCCCTAACCTTTGAGC 245 R: TCTTTTCTACTTCCCGCATACTGT 16s rRNA F: GCGGCGTGCCTAATACA 219 R: CCGCGGGTCCATCC S23 Table S3 Mass spectrum parameters Conditions Parameters Source temperature(°C) 150 Desolvent rate(L/h) 1000 Desolvent temperature(°C) 500 Parent(m/z) 790.8149 Daughter(m/z) 314.3200and 409.3026 Cone energy(V) 16 and 18 Collision energy(V) 36 and 26 ..

    Sequencing:

    Article Title: Bisphenols Promote the Pheromone-Responsive Plasmid-Mediated Conjugative Transfer of Antibiotic Resistance Genes in Enterococcus faecalis .
    Article Snippet: .. S19 S20 Figure.S10 Standard curve corresponding to the log of the copy number of each primer gene and cycle threshold (a:ccfA; b:prgQ; c:prgA; d:prgB; e:prgX; f:prgY; g:prgZ; h:pcfT; i:pcfG; j:pcfY; k:16s rRNA gene) S21 Table S1 List of bacteria and plasmids Strains or plasmids Description Antibiotic type and working concentration. pCF10 pheromone-responsive plasmid TetR(10 mg/L) pK18mobsacB Widely used gene knockout vector, KanR KanR(50 mg/L) RP4 IncPα plasmid KanR(60 mg/L), TetR(40 mg/L), AmpR(80 mg/L) OG1RS the mutant strain of E. faecalis ATCC 47077 StrR(3 g/L), RifR(50 mg/L) OG1RF(pCF10) E. faecalis ATCC 47077 containing pCF10 plasmid TetR(10 mg/L), RifR(50 mg/L) OG1RSΔccfA E. faecalis ATCC 47077 containing pCF10 plasmid ccfA deletion mutants StrR(3 g/L), RifR(50 mg/L), KanR(50 mg/L) DH5α E. coli commonly used for plasmid cloning / K12 E. coli ATCC 47076,host of RP4, RifR RifR(100 mg/L) HB101(RP4) E. coli HB101containing RP4 plasmid KanR(60 mg/L), TetR(40 mg/L), AmpR(80 mg/L) S22 Table S2 Sequence of primers Gene name Primer Sequence( 5’ to 3’) Length of the product(bp) ccfA F:AGATATACGAAGGCCCGCTGAA 243 R: AATTAGTTATCCGCTGCTCCTGA prgQ F:TATAGGAGGGGTGTAAATGAAAAC 143 R: CCGTCCTATGGCAGTCG prgA F:GTTTGATGATGGCCGCTTAGGTT R:CACTTCGCCGTTTTGGTATTGACT 175 prgB F:AGCGGATGGAAAATTTTACTCACC R: GCGCACTAGATACAGGCACATTA 197 prgX F: AATGTTTTAAATCTGAAGTA 217 R: ACAAAAAGCGTAAATGA prgY F: TTTGGAAGGTGCGGAAGAATC 202 R: GTAAATGCGCCTGCCCAATA prgZ F: GACTTTGACTGCAGGGACACC 251 R: AGCGCCATCTCTAATCACAAT pcfG F: GAACCATGGCAACTGTCTCA 179 R: GCATCCTTGTCGCTCATAAA pcfT F: GCCACTTTGCTATTATCAGGA 224 R: GTTTTCGGCTTTACAGTCTCG pcfY F: AGATAATGCCCTAACCTTTGAGC 245 R: TCTTTTCTACTTCCCGCATACTGT 16s rRNA F: GCGGCGTGCCTAATACA 219 R: CCGCGGGTCCATCC S23 Table S3 Mass spectrum parameters Conditions Parameters Source temperature(°C) 150 Desolvent rate(L/h) 1000 Desolvent temperature(°C) 500 Parent(m/z) 790.8149 Daughter(m/z) 314.3200and 409.3026 Cone energy(V) 16 and 18 Collision energy(V) 36 and 26 ..



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    e faecalis atcc 47077 strr  (ATCC)
    96
    ATCC e faecalis atcc 47077 strr
    E Faecalis Atcc 47077 Strr, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+faecalis+atcc+47077+strr/Enterococcus+faecalis%3B+OG1RF/pm36445841__es2c05349_si_001-114-78-80
    Average 96 stars, based on 1 article reviews
    e faecalis atcc 47077 strr - by Bioz Stars, 2026-10
    96/100 stars
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